Regulatory

Part:BBa_K2680524:Design

Designed by: Julia Urban   Group: iGEM18_William_and_Mary   (2018-10-12)


pLac/ara-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 425
    Illegal XhoI site found at 392
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Conserved upstream region due to comments made in Stricker paper suggesting its necessity.

Source

Sequence originally from Lutz and Bujard 1997; later used for the creation of oscillator from Stricker et al 2008.1,2

References

1Lutz, R., & Bujard, H. (1997). Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Research, 25(6), 1203–1210.

2Stricker, J., Cookson, S., Bennett, M. R., Mather, W. H., Tsimring, L. S., & Hasty, J. (2008). A fast, robust and tunable synthetic gene oscillator. Nature, 456(7221), 516–519. https://doi.org/10.1038/nature07389